[HTML][HTML] Unchanged β-adrenergic stimulation of cardiac L-type calcium channels in Cav1. 2 phosphorylation site S1928A mutant mice
T Lemke, A Welling, CJ Christel, A Blaich… - Journal of Biological …, 2008 - ASBMB
Phosphorylation of serine 1928 (Ser 1928) of the cardiac Ca v 1.2 subunit of L-type Ca 2+
channels has been proposed as the mechanism for regulation of L-type Ca 2+ channels by
protein kinase A (PKA). To test this directly in vivo, we generated a knock-in mouse with
targeted mutation of Ser 1928 to alanine. This mutation did not affect basal L-type current
characteristics or regulation of the L-type current by PKA and the β-adrenergic receptor,
whereas the mutation abolished phosphorylation of Ca v 1.2 by PKA. Therefore, our data …
channels has been proposed as the mechanism for regulation of L-type Ca 2+ channels by
protein kinase A (PKA). To test this directly in vivo, we generated a knock-in mouse with
targeted mutation of Ser 1928 to alanine. This mutation did not affect basal L-type current
characteristics or regulation of the L-type current by PKA and the β-adrenergic receptor,
whereas the mutation abolished phosphorylation of Ca v 1.2 by PKA. Therefore, our data …