Demonstration of improvements to the bioluminescence resonance energy transfer (BRET) technology for the monitoring of G protein–coupled receptors in live cells
The bioluminescence resonance energy transfer (BRET) technique has become extremely
popular for studying protein-protein interactions in living cells and real time. Of particular
interest is the ability to monitor interactions between G protein–coupled receptors, such as
the thyrotropin-releasing hormone receptor (TRHR), and proteins critical for regulating their
function, such as β-arrestin. Using TRHR/β-arrestin interactions, we have demonstrated
improvements to all 3 generations of BRET (BRET 1, BRET 2, and eBRET) by using the …
popular for studying protein-protein interactions in living cells and real time. Of particular
interest is the ability to monitor interactions between G protein–coupled receptors, such as
the thyrotropin-releasing hormone receptor (TRHR), and proteins critical for regulating their
function, such as β-arrestin. Using TRHR/β-arrestin interactions, we have demonstrated
improvements to all 3 generations of BRET (BRET 1, BRET 2, and eBRET) by using the …