[HTML][HTML] Identification and characterization of prolylcarboxypeptidase as an endothelial cell prekallikrein activator
Our recent investigations have postulated a human umbilical vein endothelial cell (HUVEC)-
associated prekallikrein activator (PKA). When prekallikrein (PK) assembles on high
molecular weight kininogen on HUVEC, PK is activated to kallikrein. PKA was found in the
15,800× g pellet of HUVEC lysates using an assay that measures PK activation only when
bound to high molecular weight kininogen linked to microtiter plates. Sequential DEAE,
wheat germ lectin affinity, and hydroxyapatite chromatography resulted in four protein bands …
associated prekallikrein activator (PKA). When prekallikrein (PK) assembles on high
molecular weight kininogen on HUVEC, PK is activated to kallikrein. PKA was found in the
15,800× g pellet of HUVEC lysates using an assay that measures PK activation only when
bound to high molecular weight kininogen linked to microtiter plates. Sequential DEAE,
wheat germ lectin affinity, and hydroxyapatite chromatography resulted in four protein bands …