Endotoxin uptake by S1 proximal tubular segment causes oxidative stress in the downstream S2 segment
R Kalakeche, T Hato, G Rhodes, KW Dunn… - Journal of the …, 2011 - journals.lww.com
Gram-negative sepsis carries high morbidity and mortality, especially when complicated by
acute kidney injury (AKI). The mechanisms of AKI in sepsis remain poorly understood. Here
we used intravital two-photon fluorescence microscopy to investigate the possibility of direct
interactions between filtered endotoxin and tubular cells as a possible mechanism of AKI in
sepsis. Using wild-type (WT), TLR4-knockout, and bone marrow chimeric mice, we found
that endotoxin is readily filtered and internalized by S1 proximal tubules through local TLR4 …
acute kidney injury (AKI). The mechanisms of AKI in sepsis remain poorly understood. Here
we used intravital two-photon fluorescence microscopy to investigate the possibility of direct
interactions between filtered endotoxin and tubular cells as a possible mechanism of AKI in
sepsis. Using wild-type (WT), TLR4-knockout, and bone marrow chimeric mice, we found
that endotoxin is readily filtered and internalized by S1 proximal tubules through local TLR4 …