[HTML][HTML] Lack of fucose on human IgG1 N-linked oligosaccharide improves binding to human FcγRIII and antibody-dependent cellular toxicity
RL Shields, J Lai, R Keck, LY O'Connell, K Hong… - Journal of Biological …, 2002 - ASBMB
Lec13 cells, a variant Chinese hamster ovary cell line, were used to produce human IgG1
that were deficient in fucose attached to the Asn 297-linked carbohydrate but were otherwise
similar to that found in IgG1 produced in normal Chinese hamster ovary cell lines and from
human serum. Lack of fucose on the IgG1 had no effect on binding to human FcγRI, C1q, or
the neonatal Fc receptor. Although no change in affinity was found for the His 131
polymorphic form of human FcγRIIA, a slight improvement in binding was evident for FcγRIIB …
that were deficient in fucose attached to the Asn 297-linked carbohydrate but were otherwise
similar to that found in IgG1 produced in normal Chinese hamster ovary cell lines and from
human serum. Lack of fucose on the IgG1 had no effect on binding to human FcγRI, C1q, or
the neonatal Fc receptor. Although no change in affinity was found for the His 131
polymorphic form of human FcγRIIA, a slight improvement in binding was evident for FcγRIIB …