Measurements of ATP in mammalian cells
G Manfredi, L Yang, CD Gajewski, M Mattiazzi - Methods, 2002 - Elsevier
G Manfredi, L Yang, CD Gajewski, M Mattiazzi
Methods, 2002•ElsevierLevels of phosphorylated adenosine nucleotides, including the universal energy carrier
adenosine 5′-triphosphate (ATP) and its metabolites adenosine 5′-diphosphate (ADP)
and adenosine 5′-monophosphate (AMP), define the energy state in living cells and are
dependent mainly on mitochondrial function. In this article, we describe a method based on
the luciferase–luciferin system used to measure mitochondrial ATP synthesis continuously in
permeabilized mammalian cells and mitochondria isolated from animal tissues. We also …
adenosine 5′-triphosphate (ATP) and its metabolites adenosine 5′-diphosphate (ADP)
and adenosine 5′-monophosphate (AMP), define the energy state in living cells and are
dependent mainly on mitochondrial function. In this article, we describe a method based on
the luciferase–luciferin system used to measure mitochondrial ATP synthesis continuously in
permeabilized mammalian cells and mitochondria isolated from animal tissues. We also …
Levels of phosphorylated adenosine nucleotides, including the universal energy carrier adenosine 5′-triphosphate (ATP) and its metabolites adenosine 5′-diphosphate (ADP) and adenosine 5′-monophosphate (AMP), define the energy state in living cells and are dependent mainly on mitochondrial function. In this article, we describe a method based on the luciferase–luciferin system used to measure mitochondrial ATP synthesis continuously in permeabilized mammalian cells and mitochondria isolated from animal tissues. We also describe a technique that uses the expression of recombinant targeted luciferase to report ATP content in different cell compartments. Finally, we describe an HPLC-based method for accurate measurement of ATP, ADP, and AMP in cultured cells and animal tissues.
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