[HTML][HTML] Constitutive dimerization of glycoprotein VI (GPVI) in resting platelets is essential for binding to collagen and activation in flowing blood
SM Jung, M Moroi, K Soejima, T Nakagaki… - Journal of Biological …, 2012 - ASBMB
The platelet collagen receptor glycoprotein VI (GPVI) has been suggested to function as a
dimer, with increased affinity for collagen. Dissociation constants (K d) obtained by
measuring recombinant GPVI binding to collagenous substrates showed that GPVI dimers
bind with high affinity to tandem GPO (Gly-Pro-Hyp) sequences in collagen, whereas the
markedly lower affinity of the monomer for all substrates implies that it is not the collagen-
binding form of GPVI. Dimer binding required a high density of immobilized triple-helical …
dimer, with increased affinity for collagen. Dissociation constants (K d) obtained by
measuring recombinant GPVI binding to collagenous substrates showed that GPVI dimers
bind with high affinity to tandem GPO (Gly-Pro-Hyp) sequences in collagen, whereas the
markedly lower affinity of the monomer for all substrates implies that it is not the collagen-
binding form of GPVI. Dimer binding required a high density of immobilized triple-helical …