Summary Background: Platelet glycoprotein (GP) VI that binds collagen, and GPIb–IX–V that
binds von Willebrand factor, initiate thrombus formation. Objectives: In this study, we
investigated the mechanisms of metalloproteinase‐mediated ectodomain shedding that
regulate the surface expression of GPVI, GPIbα (the major ligand‐binding subunit) and GPV
(that regulates thrombin‐dependent activation via GPIbα). Methods and results:
Immunoblotting human platelet lysates using affinity‐purified antibodies against cytoplasmic …