Ribosome profiling reveals the what, when, where and how of protein synthesis

GA Brar, JS Weissman - Nature reviews Molecular cell biology, 2015 - nature.com
Nature reviews Molecular cell biology, 2015nature.com
Ribosome profiling, which involves the deep sequencing of ribosome-protected mRNA
fragments, is a powerful tool for globally monitoring translation in vivo. The method has
facilitated discovery of the regulation of gene expression underlying diverse and complex
biological processes, of important aspects of the mechanism of protein synthesis, and even
of new proteins, by providing a systematic approach for experimental annotation of coding
regions. Here, we introduce the methodology of ribosome profiling and discuss examples in …
Abstract
Ribosome profiling, which involves the deep sequencing of ribosome-protected mRNA fragments, is a powerful tool for globally monitoring translation in vivo. The method has facilitated discovery of the regulation of gene expression underlying diverse and complex biological processes, of important aspects of the mechanism of protein synthesis, and even of new proteins, by providing a systematic approach for experimental annotation of coding regions. Here, we introduce the methodology of ribosome profiling and discuss examples in which this approach has been a key factor in guiding biological discovery, including its prominent role in identifying thousands of novel translated short open reading frames and alternative translation products.
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