Two-photon intravital fluorescence lifetime imaging of the kidney reveals cell-type specific metabolic signatures

T Hato, S Winfree, R Day, RM Sandoval… - Journal of the …, 2017 - journals.lww.com
In the live animal, tissue autofluorescence arises from a number of biologically important
metabolites, such as the reduced form of nicotinamide adenine dinucleotide. Because
autofluorescence changes with metabolic state, it can be harnessed as a label-free imaging
tool with which to study metabolism in vivo. Here, we used the combination of intravital two-
photon microscopy and frequency-domain fluorescence lifetime imaging microscopy (FLIM)
to map cell-specific metabolic signatures in the kidneys of live animals. The FLIM images are …