[HTML][HTML] α-Hemoglobin stabilizing protein (AHSP) markedly decreases the redox potential and reactivity of α-subunits of human HbA with hydrogen peroxide
TL Mollan, S Banerjee, G Wu, CJP Siburt… - Journal of Biological …, 2013 - ASBMB
α-Hemoglobin stabilizing protein (AHSP) is a molecular chaperone that binds monomeric α-
subunits of human hemoglobin A (HbA) and modulates heme iron oxidation and subunit
folding states. Although AHSP· αHb complexes autoxidize more rapidly than HbA, the redox
mechanisms appear to be similar. Both metHbA and isolated met-β-subunits undergo further
oxidation in the presence of hydrogen peroxide (H 2 O 2) to form ferryl heme species.
Surprisingly, much lower levels of H 2 O 2-induced ferryl heme are produced by free met-α …
subunits of human hemoglobin A (HbA) and modulates heme iron oxidation and subunit
folding states. Although AHSP· αHb complexes autoxidize more rapidly than HbA, the redox
mechanisms appear to be similar. Both metHbA and isolated met-β-subunits undergo further
oxidation in the presence of hydrogen peroxide (H 2 O 2) to form ferryl heme species.
Surprisingly, much lower levels of H 2 O 2-induced ferryl heme are produced by free met-α …