2-Methoxyestradiol regulates VEGFR-2 and sFlt-1 expression in human placenta

DK Lee, O Nevo - Placenta, 2015 - Elsevier
Abstract Introduction 2-Methoxyestradiol (2-ME), a metabolite of estradiol, has been
identified as an initiator of cytotrophoblast transformation to an invasive phenotype, with low
levels implicated with the onset of preeclampsia. Here, we investigated the effects of 2-ME
on VEGFR-2, sFlt-1 and HIF1α expression in human placenta. Methods First trimester
human placental villous explants were maintained at 3% and 20% O 2. Samples were
treated with 0.5 μM 2-ME with or without 1 mM DMOG or 0.2 mM CoCl 2 for 17 h. Western …