Restricted varicella-zoster virus transcription in human trigeminal ganglia obtained soon after death
WJD Ouwendijk, A Choe, MA Nagel, D Gilden… - Journal of …, 2012 - Am Soc Microbiol
WJD Ouwendijk, A Choe, MA Nagel, D Gilden, ADME Osterhaus, RJ Cohrs, GMGM Verjans
Journal of virology, 2012•Am Soc MicrobiolWe analyzed the varicella-zoster virus (VZV) transcriptome in 43 latently infected human
trigeminal ganglia (TG) with postmortem intervals (PMIs) ranging from 3.7 to 24 h. Multiplex
reverse transcriptase PCR (RT-PCR) revealed no VZV transcripts with a PMI of< 9 h. Real-
time PCR indicated a significant increase (P= 0.02) in VZV ORF63 transcript levels but not
the virus DNA burden with longer PMI. Overall, both the breadth of the VZV transcriptome
and the VZV ORF63 transcript levels in human cadaver TG increased with longer PMI.
trigeminal ganglia (TG) with postmortem intervals (PMIs) ranging from 3.7 to 24 h. Multiplex
reverse transcriptase PCR (RT-PCR) revealed no VZV transcripts with a PMI of< 9 h. Real-
time PCR indicated a significant increase (P= 0.02) in VZV ORF63 transcript levels but not
the virus DNA burden with longer PMI. Overall, both the breadth of the VZV transcriptome
and the VZV ORF63 transcript levels in human cadaver TG increased with longer PMI.
Abstract
We analyzed the varicella-zoster virus (VZV) transcriptome in 43 latently infected human trigeminal ganglia (TG) with postmortem intervals (PMIs) ranging from 3.7 to 24 h. Multiplex reverse transcriptase PCR (RT-PCR) revealed no VZV transcripts with a PMI of <9 h. Real-time PCR indicated a significant increase (P = 0.02) in VZV ORF63 transcript levels but not the virus DNA burden with longer PMI. Overall, both the breadth of the VZV transcriptome and the VZV ORF63 transcript levels in human cadaver TG increased with longer PMI.
American Society for Microbiology