Identification of 3H-Naphtho[1,2,3-de]quinoline-2,7-diones as Inhibitors of Apoptosis Signal-Regulating Kinase 1 (ASK1)
Journal of medicinal chemistry, 2011•ACS Publications
Apoptosis signal-regulating kinase 1 (ASK1) has recently emerged as an attractive
therapeutic target for the treatment of cardiac and neurodegenerative disorders. The
selective inhibitors of ASK1 may become important compounds for the development of
clinical agents. We have identified the ASK1 inhibitor among 3 H-naphtho [1, 2, 3-de]
quinoline-2, 7-diones using receptor-based virtual screening. In vitro kinase assay revealed
that ethyl 2, 7-dioxo-2, 7-dihydro-3 H-naphtho [1, 2, 3-de] quinoline-1-carboxylate (NQDI-1) …
therapeutic target for the treatment of cardiac and neurodegenerative disorders. The
selective inhibitors of ASK1 may become important compounds for the development of
clinical agents. We have identified the ASK1 inhibitor among 3 H-naphtho [1, 2, 3-de]
quinoline-2, 7-diones using receptor-based virtual screening. In vitro kinase assay revealed
that ethyl 2, 7-dioxo-2, 7-dihydro-3 H-naphtho [1, 2, 3-de] quinoline-1-carboxylate (NQDI-1) …
Apoptosis signal-regulating kinase 1 (ASK1) has recently emerged as an attractive therapeutic target for the treatment of cardiac and neurodegenerative disorders. The selective inhibitors of ASK1 may become important compounds for the development of clinical agents. We have identified the ASK1 inhibitor among 3H-naphtho[1,2,3-de]quinoline-2,7-diones using receptor-based virtual screening. In vitro kinase assay revealed that ethyl 2,7-dioxo-2,7-dihydro-3H-naphtho[1,2,3-de]quinoline-1-carboxylate (NQDI-1) inhibited ASK1 with a Ki of 500 nM. The competitive character of inhibition is demonstrated in Lineweaver−Burk plots. In our preliminary selectivity study this compound exhibited strong specific inhibitory activity toward ASK1.
ACS Publications