[HTML][HTML] Macrophages with a deletion of the phosphoenolpyruvate carboxykinase 1 (Pck1) gene have a more proinflammatory phenotype
Phosphoenolpyruvate carboxykinase (Pck1) is a metabolic enzyme that is integral to the
gluconeogenic and glyceroneogenic pathways. However, Pck1's role in macrophage
metabolism and function is unknown. Using stable isotopomer MS analysis in a mouse
model with a myeloid cell–specific Pck1 deletion, we show here that this deletion increases
the proinflammatory phenotype in macrophages. Incubation of LPS-stimulated bone marrow–
derived macrophages (BMDM) with [U-13 C] glucose revealed reduced 13 C labeling of …
gluconeogenic and glyceroneogenic pathways. However, Pck1's role in macrophage
metabolism and function is unknown. Using stable isotopomer MS analysis in a mouse
model with a myeloid cell–specific Pck1 deletion, we show here that this deletion increases
the proinflammatory phenotype in macrophages. Incubation of LPS-stimulated bone marrow–
derived macrophages (BMDM) with [U-13 C] glucose revealed reduced 13 C labeling of …