Development and validation of a multiplex immunoassay for the simultaneous determination of serum antibodies to Bordetella pertussis, diphtheria and tetanus

PGM van Gageldonk, FG van Schaijk… - Journal of …, 2008 - Elsevier
To increase testing of vaccine induced humoral immunity in immune surveillance studies
and vaccine trials, a rapid and simple microsphere-based multiplex assay (pentaplex) was
developed for the quantitation of IgG serum antibodies directed against the Bordetella
pertussis antigens: Pertussis Toxin (Ptx), Filamentous hemagglutinin (FHA), Pertactin (Prn)
and to Diphtheria toxin and Tetanus toxin. All individual antigens were covalently linked to
carboxylated microspheres. The method was validated with different serum panels (n= 60 …