Simultaneous generation of many RNA-seq libraries in a single reaction
AA Shishkin, G Giannoukos, A Kucukural, D Ciulla… - Nature …, 2015 - nature.com
Nature methods, 2015•nature.com
Although RNA-seq is a powerful tool, the considerable time and cost associated with library
construction has limited its utilization for various applications. RNAtag-Seq, an approach to
generate multiple RNA-seq libraries in a single reaction, lowers time and cost per sample,
and it produces data on prokaryotic and eukaryotic samples that are comparable to those
generated by traditional strand-specific RNA-seq approaches.
construction has limited its utilization for various applications. RNAtag-Seq, an approach to
generate multiple RNA-seq libraries in a single reaction, lowers time and cost per sample,
and it produces data on prokaryotic and eukaryotic samples that are comparable to those
generated by traditional strand-specific RNA-seq approaches.
Abstract
Although RNA-seq is a powerful tool, the considerable time and cost associated with library construction has limited its utilization for various applications. RNAtag-Seq, an approach to generate multiple RNA-seq libraries in a single reaction, lowers time and cost per sample, and it produces data on prokaryotic and eukaryotic samples that are comparable to those generated by traditional strand-specific RNA-seq approaches.
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