[HTML][HTML] A method for culturing Gram-negative skin microbiota
IA Myles, JD Reckhow, KW Williams, I Sastalla… - BMC microbiology, 2016 - Springer
IA Myles, JD Reckhow, KW Williams, I Sastalla, KM Frank, SK Datta
BMC microbiology, 2016•SpringerAbstract Background Commensal Gram-negative (CGN) microbiota have been identified on
human skin by DNA sequencing; however, methods to reliably culture viable Gram-negative
skin organisms have not been previously described. Results Through the use of selective
antibiotics and minimal media we developed methods to culture CGN from skin swabs. We
identified several previously uncharacterized CGN at the species level by optimizing growth
conditions and limiting the inhibitory effects of nutrient shock, temperature, and bacterial …
human skin by DNA sequencing; however, methods to reliably culture viable Gram-negative
skin organisms have not been previously described. Results Through the use of selective
antibiotics and minimal media we developed methods to culture CGN from skin swabs. We
identified several previously uncharacterized CGN at the species level by optimizing growth
conditions and limiting the inhibitory effects of nutrient shock, temperature, and bacterial …
Background
Commensal Gram-negative (CGN) microbiota have been identified on human skin by DNA sequencing; however, methods to reliably culture viable Gram-negative skin organisms have not been previously described.
Results
Through the use of selective antibiotics and minimal media we developed methods to culture CGN from skin swabs. We identified several previously uncharacterized CGN at the species level by optimizing growth conditions and limiting the inhibitory effects of nutrient shock, temperature, and bacterial competition, factors that may have previously limited CGN isolation from skin cultures.
Conclusions
Our protocol will permit future functional studies on the influences of CGN on skin homeostasis and disease.
Springer