Measurement of myeloid cell immune suppressive activity
L Dolcetti, E Peranzoni, V Bronte - Current protocols in …, 2010 - Wiley Online Library
L Dolcetti, E Peranzoni, V Bronte
Current protocols in immunology, 2010•Wiley Online LibraryThis unit presents simple methods to assess the immunosuppressive properties of
immunoregulatory cells of myeloid origin, such as myeloid‐derived suppressor cells
(MDSCs), both in vitro and in vivo. These methods are general and could be adapted to test
the impact of different suppressive populations on T cell activation, proliferation, and
cytotoxic activity; moreover they could be useful to assess the influence exerted on immune
suppressive pathways by genetic modifications, chemical inhibitors, and drugs. Curr. Protoc …
immunoregulatory cells of myeloid origin, such as myeloid‐derived suppressor cells
(MDSCs), both in vitro and in vivo. These methods are general and could be adapted to test
the impact of different suppressive populations on T cell activation, proliferation, and
cytotoxic activity; moreover they could be useful to assess the influence exerted on immune
suppressive pathways by genetic modifications, chemical inhibitors, and drugs. Curr. Protoc …
Abstract
This unit presents simple methods to assess the immunosuppressive properties of immunoregulatory cells of myeloid origin, such as myeloid‐derived suppressor cells (MDSCs), both in vitro and in vivo. These methods are general and could be adapted to test the impact of different suppressive populations on T cell activation, proliferation, and cytotoxic activity; moreover they could be useful to assess the influence exerted on immune suppressive pathways by genetic modifications, chemical inhibitors, and drugs. Curr. Protoc. Immunol. 91:14.17.1‐14.17.25. © 2010 by John Wiley & Sons, Inc.
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