[HTML][HTML] Arginine 104 is a key catalytic residue in leukotriene C4 synthase
A Rinaldo-Matthis, A Wetterholm, DM Molina… - Journal of biological …, 2010 - ASBMB
Human leukotriene C 4 synthase (hLTC 4 S) is an integral membrane enzyme that
conjugates leukotriene (LT) A 4 with glutathione to form LTC 4, a precursor to the cysteinyl
leukotrienes (LTC 4, LTD 4, and LTE 4) that are involved in the pathogenesis of human
bronchial asthma. From the crystal structure of hLTC 4 S, Arg-104 and Arg-31 have been
implicated in the conjugation reaction. Here, we used site-directed mutagenesis, UV
spectroscopy, and x-ray crystallography to examine the catalytic role of Arg-104 and Arg-31 …
conjugates leukotriene (LT) A 4 with glutathione to form LTC 4, a precursor to the cysteinyl
leukotrienes (LTC 4, LTD 4, and LTE 4) that are involved in the pathogenesis of human
bronchial asthma. From the crystal structure of hLTC 4 S, Arg-104 and Arg-31 have been
implicated in the conjugation reaction. Here, we used site-directed mutagenesis, UV
spectroscopy, and x-ray crystallography to examine the catalytic role of Arg-104 and Arg-31 …