Characterization of the leukotriene D4 receptor in dimethylsulphoxide-differentiated U937 cells: comparison with the leukotriene D4 receptor in human lung and …
EA Frey, DW Nicholson, KM Metters - European Journal of Pharmacology …, 1993 - Elsevier
The leukotriene D 4 receptor has been fully characterized by radioligand binding in
membrane preparations from dimethyl sulphoxide-differentiated U937 cells, a human
monocyte leukemia cell line, and, in parallel experiments, compared with leukotriene D 4
receptor found in human lung and guinea-pig lung preparations.[3 H] Leukotriene D 4
specific binding in differentiated U937 cell membranes is of high affinity (KD= 0.35 nM),
saturable (B max= 287 fmol/mg protein), with differentiation resulting in a 3–5-fold increase …
membrane preparations from dimethyl sulphoxide-differentiated U937 cells, a human
monocyte leukemia cell line, and, in parallel experiments, compared with leukotriene D 4
receptor found in human lung and guinea-pig lung preparations.[3 H] Leukotriene D 4
specific binding in differentiated U937 cell membranes is of high affinity (KD= 0.35 nM),
saturable (B max= 287 fmol/mg protein), with differentiation resulting in a 3–5-fold increase …