A comparison of TRECs and flow cytometry for naive T cell quantification
SP Adams, S Kricke, E Ralph, N Gilmour… - Clinical & …, 2018 - academic.oup.com
SP Adams, S Kricke, E Ralph, N Gilmour, KC Gilmour
Clinical & Experimental Immunology, 2018•academic.oup.comAssessment of thymic output by measurement of naive T cells is carried out routinely in
clinical diagnostic laboratories, predominantly using flow cytometry with a suitable panel of
antibodies. Naive T cell measurements can also be made using molecular analyses to
quantify T cell receptor excision circle (TRECs) levels in sorted cells from peripheral blood.
In this study we have compared TRECs levels retrospectively with CD45RA+ CD27+ T cells
and also with CD45RA+ CD31+ T cells in 134 patient samples at diagnosis or during follow …
clinical diagnostic laboratories, predominantly using flow cytometry with a suitable panel of
antibodies. Naive T cell measurements can also be made using molecular analyses to
quantify T cell receptor excision circle (TRECs) levels in sorted cells from peripheral blood.
In this study we have compared TRECs levels retrospectively with CD45RA+ CD27+ T cells
and also with CD45RA+ CD31+ T cells in 134 patient samples at diagnosis or during follow …
Summary
Assessment of thymic output by measurement of naive T cells is carried out routinely in clinical diagnostic laboratories, predominantly using flow cytometry with a suitable panel of antibodies. Naive T cell measurements can also be made using molecular analyses to quantify T cell receptor excision circle (TRECs) levels in sorted cells from peripheral blood. In this study we have compared TRECs levels retrospectively with CD45RA+CD27+ T cells and also with CD45RA+CD31+ T cells in 134 patient samples at diagnosis or during follow-up. Both panels provide naive T cell measurements that have a strongly positive correlation with TRECs numbers and are suitable for use with enumerating naive T cell levels in a clinical laboratory.
Oxford University Press