Robust enumeration of cell subsets from tissue expression profiles

AM Newman, CL Liu, MR Green, AJ Gentles, W Feng… - Nature …, 2015 - nature.com
AM Newman, CL Liu, MR Green, AJ Gentles, W Feng, Y Xu, CD Hoang, M Diehn
Nature methods, 2015nature.com
We introduce CIBERSORT, a method for characterizing cell composition of complex tissues
from their gene expression profiles. When applied to enumeration of hematopoietic subsets
in RNA mixtures from fresh, frozen and fixed tissues, including solid tumors, CIBERSORT
outperformed other methods with respect to noise, unknown mixture content and closely
related cell types. CIBERSORT should enable large-scale analysis of RNA mixtures for
cellular biomarkers and therapeutic targets (http://cibersort. stanford. edu/).
Abstract
We introduce CIBERSORT, a method for characterizing cell composition of complex tissues from their gene expression profiles. When applied to enumeration of hematopoietic subsets in RNA mixtures from fresh, frozen and fixed tissues, including solid tumors, CIBERSORT outperformed other methods with respect to noise, unknown mixture content and closely related cell types. CIBERSORT should enable large-scale analysis of RNA mixtures for cellular biomarkers and therapeutic targets (http://cibersort.stanford.edu/).
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