[HTML][HTML] Induction of pyruvate dehydrogenase kinase-3 by hypoxia-inducible factor-1 promotes metabolic switch and drug resistance
The switch of cellular metabolism from mitochondrial respiration to glycolysis is the hallmark
of cancer cells and associated with tumor malignancy. However, the mechanism of this
metabolic switch remains largely unknown. Herein, we reported that hypoxia-inducible factor-
1 (HIF-1) induced pyruvate dehydrogenase kinase-3 (PDK3) expression leading to inhibition
of mitochondrial respiration. Promoter activity assay, small interference RNA knockdown
assay, and chromatin immunoprecipitation assay demonstrated that hypoxia-induced PDK3 …
of cancer cells and associated with tumor malignancy. However, the mechanism of this
metabolic switch remains largely unknown. Herein, we reported that hypoxia-inducible factor-
1 (HIF-1) induced pyruvate dehydrogenase kinase-3 (PDK3) expression leading to inhibition
of mitochondrial respiration. Promoter activity assay, small interference RNA knockdown
assay, and chromatin immunoprecipitation assay demonstrated that hypoxia-induced PDK3 …