Paracrine control of Leydig cell activity by FSH dependent proteins from Sertoli cells: an in vitro study

M Benahmed, E Tabone, C Grenot, P Sanchez… - Journal of Steroid …, 1986 - Elsevier
The regulating effect of follicle-stimulating hormone (FSH) on Leydig cell function was
studied using a model of immature porcine Leydig and Sertoli cells cultured in a hormone
supplemented defined medium. FSH pretreatment for 2 days of Leydig cells cultured alone
was with no effect. FSH pretreatment of Leydig cells cocultured with Sertoli cells increases
Leydig cell activity in an FSH dose-dependent manner with a maximal effect observed at 50
ng/ml porcine FSH (pFSH). Leydig cells cultured for 2 days in conditioned medium (CM) by …