[HTML][HTML] Ligand-induced dynamic membrane changes and cell deletion conferred by vanilloid receptor 1
Z Olah, T Szabo, L Karai, C Hough, RD Fields… - Journal of Biological …, 2001 - ASBMB
The real time dynamics of vanilloid-induced cytotoxicity and the specific deletion of
nociceptive neurons expressing the wild-type vanilloid receptor (VR1) were investigated.
VR1 was C-terminally tagged with either the 27-kDa enhanced green fluorescent protein
(eGFP) or a 12-amino acid ε-epitope. Upon exposure to resiniferatoxin, VR1eGFP-or VR1ε-
expressing cells exhibited pharmacological responses similar to those of cells expressing
the untagged VR1. Within seconds of vanilloid exposure, the intracellular free calcium ([Ca …
nociceptive neurons expressing the wild-type vanilloid receptor (VR1) were investigated.
VR1 was C-terminally tagged with either the 27-kDa enhanced green fluorescent protein
(eGFP) or a 12-amino acid ε-epitope. Upon exposure to resiniferatoxin, VR1eGFP-or VR1ε-
expressing cells exhibited pharmacological responses similar to those of cells expressing
the untagged VR1. Within seconds of vanilloid exposure, the intracellular free calcium ([Ca …