[HTML][HTML] Quantification of female and male Plasmodium falciparum gametocytes by reverse transcriptase quantitative PCR
P Schneider, SE Reece, BCL Van Schaijk… - Molecular and …, 2015 - Elsevier
The transmission of malaria parasites depends on the presence of sexual stages
(gametocytes) in the blood, making the ratio and densities of female and male gametocytes
important determinants of parasite fitness. This manuscript describes the development of
reverse transcriptase quantitative PCR (RT-qPCR) assays to separately quantify mature
female and male gametocytes of the human malaria parasite Plasmodium falciparum, and
reveals that Pfs25 mRNA is expressed only in female gametocytes. The female (Pfs25) and …
(gametocytes) in the blood, making the ratio and densities of female and male gametocytes
important determinants of parasite fitness. This manuscript describes the development of
reverse transcriptase quantitative PCR (RT-qPCR) assays to separately quantify mature
female and male gametocytes of the human malaria parasite Plasmodium falciparum, and
reveals that Pfs25 mRNA is expressed only in female gametocytes. The female (Pfs25) and …