Monocyte chemoattractant protein-1 is produced in isolated adipocytes, associated with adiposity and reduced after weight loss in morbid obese subjects

T Christiansen, B Richelsen, JM Bruun - International journal of obesity, 2005 - nature.com
T Christiansen, B Richelsen, JM Bruun
International journal of obesity, 2005nature.com
BACKGROUND: Obesity is associated with insulin resistance and premature
atherosclerosis. The human adipose tissue produce several adipokines including monocyte
chemoattractant protein (MCP)-1, associated with cardiovascular disease and found to be
involved in the pathogenesis of atherosclerosis in vitro. OBJECTIVE:(1) To compare mRNA
levels of MCP-1, leptin and a macrophage-specific marker (CD68) in isolated adipocytes vs
stromal–vascular (SV) cells,(2) to compare mRNA levels of MCP-1 in human adipose tissue …
Abstract
BACKGROUND:
Obesity is associated with insulin resistance and premature atherosclerosis. The human adipose tissue produce several adipokines including monocyte chemoattractant protein (MCP)-1, associated with cardiovascular disease and found to be involved in the pathogenesis of atherosclerosis in vitro.
OBJECTIVE:
(1) To compare mRNA levels of MCP-1, leptin and a macrophage-specific marker (CD68) in isolated adipocytes vs stromal–vascular (SV) cells,(2) to compare mRNA levels of MCP-1 in human adipose tissue to circulating MCP-1 and adiposity (eg BMI: kg/m 2) and (3) investigate the effect of weight loss in obese subjects on circulating MCP-1 and leptin.
RESULTS:
(1) MCP-1 and CD68 mRNA levels in isolated adipocytes vs SV cells were 17%(P< 0.01) and∼ 2%(P< 0.001), respectively. Leptin mRNA levels in SV cells were∼ 1% of that in isolated adipocytes (P< 0.01).(2) MCP-1 mRNA levels correlated with circulating MCP-1 (P< 0.05) and BMI (P< 0.05).(3) A 12% weight loss (P< 0.001) was associated with a 25% decrease in insulin levels (P< 0.01). Circulating MCP-1 and leptin decreased by 20%(P< 0.001) and by 24%(P< 0.001), respectively.
DISCUSSION:
The findings demonstrate that MCP-1 is produced in isolated human adipocytes. In addition, the findings suggest that MCP-1 may be involved in obesity-related health complications and support the hypothesis that weight loss is beneficial by improving the low-grade inflammation observed in obesity.
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