Assessment of protein C anticoagulant pathway by thrombin generation assay in the presence of endothelial cells
E Coll, L Robles‐Carrillo, E Reyes… - Journal of Thrombosis …, 2013 - jthjournal.org
The generation of thrombin is the pivotal event in the process of blood coagulation. In vivo,
thrombin generation is regulated by cooperation between the vascular endothelium and the
procoagulant and anticoagulant systems in blood, such as the thrombin/endothelial cell–
dependent activation of the protein C anticoagulant pathway that ultimately leads to factor
Va (FVa) and FVIIIa inactivation [1, 2]. In vitro, thrombin generation is assessed most
commonly in plasma by use of a fluorogenic substrate–based assay (TGA) in microtiter …
thrombin generation is regulated by cooperation between the vascular endothelium and the
procoagulant and anticoagulant systems in blood, such as the thrombin/endothelial cell–
dependent activation of the protein C anticoagulant pathway that ultimately leads to factor
Va (FVa) and FVIIIa inactivation [1, 2]. In vitro, thrombin generation is assessed most
commonly in plasma by use of a fluorogenic substrate–based assay (TGA) in microtiter …
