Enhanced apoptosis in prolonged cultures of senescent porcine pulmonary artery endothelial cells
J Zhang, JM Patel, ER Block - Mechanisms of ageing and development, 2002 - Elsevier
Senescent or aged endothelial cells in culture remain metabolically active after cessation of
division, and are generally believed to eventually die. However, mechanisms underlying the
terminal aging of cultured cells, ie from senescence to death, are poorly understood. Here,
we report that culturing of replicative senescent endothelial cells for a prolonged period of
time without passaging leads to enhanced programmed cell death or apoptosis. Senescent
(passage 45) and young (passage 3) porcine pulmonary artery endothelial cells (PAEC) …
division, and are generally believed to eventually die. However, mechanisms underlying the
terminal aging of cultured cells, ie from senescence to death, are poorly understood. Here,
we report that culturing of replicative senescent endothelial cells for a prolonged period of
time without passaging leads to enhanced programmed cell death or apoptosis. Senescent
(passage 45) and young (passage 3) porcine pulmonary artery endothelial cells (PAEC) …