Within adipose tissue, multiple leukocyte interactions contribute to metabolic homeostasis in
health as well as to the pathogenesis of insulin resistance with obesity. Adipose tissue
macrophages (ATMs) are the predominant leukocyte population in fat and contribute to
obesity-induced inflammation. Characterization of ATMs and other leukocytes in the stromal
vascular fraction from fat has benefited from the use of flow cytometry and flow-assisted cell
sorting techniques. These methods permit the immunophenotyping, quantification, and …