[HTML][HTML] Reducing the environmental sensitivity of yellow fluorescent protein: mechanism and applications
Yellow mutants of the green fluorescent protein (YFP) are crucial constituents of genetically
encoded indicators of signal transduction and fusions to monitor protein-protein interactions.
However, previous YFPs show excessive pH sensitivity, chloride interference, poor
photostability, or poor expression at 37° C. Protein evolution in Escherichia coli has
produced a new YFP named Citrine, in which the mutation Q69M confers a much lower pK a
(5.7) than for previous YFPs, indifference to chloride, twice the photostability of previous …
encoded indicators of signal transduction and fusions to monitor protein-protein interactions.
However, previous YFPs show excessive pH sensitivity, chloride interference, poor
photostability, or poor expression at 37° C. Protein evolution in Escherichia coli has
produced a new YFP named Citrine, in which the mutation Q69M confers a much lower pK a
(5.7) than for previous YFPs, indifference to chloride, twice the photostability of previous …