Blue native polyacrylamide gel electrophoresis (BN-PAGE) for analysis of multiprotein complexes from cellular lysates
GJ Fiala, WWA Schamel, B Blumenthal - JoVE (Journal of Visualized …, 2011 - jove.com
Multiprotein complexes (MPCs) play a crucial role in cell signalling, since most proteins can
be found in functional or regulatory complexes with other proteins (Sali, Glaeser et al. 2003).
Thus, the study of protein-protein interaction networks requires the detailed characterization
of MPCs to gain an integrative understanding of protein function and regulation. For
identification and analysis, MPCs must be separated under native conditions. In this video,
we describe the analysis of MPCs by blue native polyacrylamide gel electrophoresis (BN …
be found in functional or regulatory complexes with other proteins (Sali, Glaeser et al. 2003).
Thus, the study of protein-protein interaction networks requires the detailed characterization
of MPCs to gain an integrative understanding of protein function and regulation. For
identification and analysis, MPCs must be separated under native conditions. In this video,
we describe the analysis of MPCs by blue native polyacrylamide gel electrophoresis (BN …