Depletion of polyubiquitin encoded by the UBI4 gene confers pleiotropic phenotype to Candida albicans cells
P Roig, D Gozalbo - Fungal Genetics and Biology, 2003 - Elsevier
We have studied the roles of polyubiquitin in Candida albicans physiology. Heterologous
expression of the C. albicans polyubiquitin (UBI4) gene in a ubi4 Saccharomyces cerevisiae
strain suppressed the mutant phenotype (hypersensitivity to heat shock). A heterozygous
strain UBI4/Δubi4:: hisG, obtained following the ura-blaster procedure, was used to construct
a conditional mutant using a pCaDis derivative plasmid. By serendipity we isolated the UBI4
conditional mutant as well as a UBI4 mutant containing a non-functional MET3 promoter …
expression of the C. albicans polyubiquitin (UBI4) gene in a ubi4 Saccharomyces cerevisiae
strain suppressed the mutant phenotype (hypersensitivity to heat shock). A heterozygous
strain UBI4/Δubi4:: hisG, obtained following the ura-blaster procedure, was used to construct
a conditional mutant using a pCaDis derivative plasmid. By serendipity we isolated the UBI4
conditional mutant as well as a UBI4 mutant containing a non-functional MET3 promoter …