[HTML][HTML] Visualizing PU. 1 activity during hematopoiesis
OBJECTIVE: PU. 1 is a critical transcription factor for hematopoietic development that is
required for the early differentiation of myeloid, erythroid, and B lineage cells. To gain a
better insight into PU. 1 function, we performed a comprehensive analysis of PU. 1 gene
activity in the hematopoietic system, using a green fluorescent protein reporter mouse line.
METHODS: We used flow cytometry to analyze green fluorescent protein (GFP) expression,
along with various cell surface markers, in heterozygote mice that harbor a GFP reporter …
required for the early differentiation of myeloid, erythroid, and B lineage cells. To gain a
better insight into PU. 1 function, we performed a comprehensive analysis of PU. 1 gene
activity in the hematopoietic system, using a green fluorescent protein reporter mouse line.
METHODS: We used flow cytometry to analyze green fluorescent protein (GFP) expression,
along with various cell surface markers, in heterozygote mice that harbor a GFP reporter …