A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals.
DR Burton, CF Barbas 3rd… - Proceedings of the …, 1991 - National Acad Sciences
DR Burton, CF Barbas 3rd, MA Persson, S Koenig, RM Chanock, RA Lerner
Proceedings of the National Academy of Sciences, 1991•National Acad SciencesA panel of human monoclonal antibody Fab fragments has been generated against the
surface glycoprotein gp120 of type 1 human immunodeficiency virus (HIV) by antigen
selection from a random combinatorial library expressed on the surface of filamentous
phage. The library was prepared from 5 ml of bone marrow from an asymptomatic individual
who has been HIV-positive for 6 years. The antibodies have high affinity for antigen (mostly
with affinity constants of greater than 10 (8) M-1) and notable sequence diversity. Given …
surface glycoprotein gp120 of type 1 human immunodeficiency virus (HIV) by antigen
selection from a random combinatorial library expressed on the surface of filamentous
phage. The library was prepared from 5 ml of bone marrow from an asymptomatic individual
who has been HIV-positive for 6 years. The antibodies have high affinity for antigen (mostly
with affinity constants of greater than 10 (8) M-1) and notable sequence diversity. Given …
A panel of human monoclonal antibody Fab fragments has been generated against the surface glycoprotein gp120 of type 1 human immunodeficiency virus (HIV) by antigen selection from a random combinatorial library expressed on the surface of filamentous phage. The library was prepared from 5 ml of bone marrow from an asymptomatic individual who has been HIV-positive for 6 years. The antibodies have high affinity for antigen (mostly with affinity constants of greater than 10(8) M-1) and notable sequence diversity. Given appropriate donor selection, the methods described should allow the generation of antibodies for the evaluation of passive immunization as a therapy for AIDS.
National Acad Sciences