Reducing acetate excretion from E. coli K-12 by over-expressing the small RNA SgrS
A Negrete, N Majdalani, JN Phue, J Shiloach - New biotechnology, 2013 - Elsevier
When exposed to the nonmetabolized glucose derivative alpha methyl glucoside (αMG),
both Escherichia coli K-12 (JM109 and MG1655) and E. coli B (BL21) respond by reducing
the concentration of the mRNA of the ptsG gene which is responsible for the biosynthesis of
the glucose transporter EIICBglu. This occurs through the over-expression of the noncoding
small RNA SgrS, which interacts specifically with the mRNA of the ptsG gene and prevents
its translation. However, when these bacteria are exposed to a glucose concentration of …
both Escherichia coli K-12 (JM109 and MG1655) and E. coli B (BL21) respond by reducing
the concentration of the mRNA of the ptsG gene which is responsible for the biosynthesis of
the glucose transporter EIICBglu. This occurs through the over-expression of the noncoding
small RNA SgrS, which interacts specifically with the mRNA of the ptsG gene and prevents
its translation. However, when these bacteria are exposed to a glucose concentration of …