Purpose A quantitative study was conducted to estimate the survival time of giant vacuoles
of the endothelial wall of Schlemm's canal once perfusion pressure has dropped to zero.
Vacuoles are known to be sensitive to intraocular pressure and could serve as real-time
markers of aqueous pressure in the juxtacanalicular tissue if their survival time is short once
pressure is lowered. Methods Six pairs of healthy human eyes were perfused with
phosphate-buffered saline. One eye was then fixed by perfusion, whereas the fellow eye …