Relating conformation to function in integrin α5β1

Y Su, W Xia, J Li, T Walz… - Proceedings of the …, 2016 - National Acad Sciences
Proceedings of the National Academy of Sciences, 2016National Acad Sciences
Whether β1 integrin ectodomains visit conformational states similarly to β2 and β3 integrins
has not been characterized. Furthermore, despite a wealth of activating and inhibitory
antibodies to β1 integrins, the conformational states that these antibodies stabilize, and the
relation of these conformations to function, remain incompletely characterized. Using
negative-stain electron microscopy, we show that the integrin α5β1 ectodomain adopts
extended-closed and extended-open conformations as well as a bent conformation …
Whether β1 integrin ectodomains visit conformational states similarly to β2 and β3 integrins has not been characterized. Furthermore, despite a wealth of activating and inhibitory antibodies to β1 integrins, the conformational states that these antibodies stabilize, and the relation of these conformations to function, remain incompletely characterized. Using negative-stain electron microscopy, we show that the integrin α5β1 ectodomain adopts extended-closed and extended-open conformations as well as a bent conformation. Antibodies SNAKA51, 8E3, N29, and 9EG7 bind to different domains in the α5 or β1 legs, activate, and stabilize extended ectodomain conformations. Antibodies 12G10 and HUTS-4 bind to the β1 βI domain and hybrid domains, respectively, activate, and stabilize the open headpiece conformation. Antibody TS2/16 binds a similar epitope as 12G10, activates, and appears to stabilize an open βI domain conformation without requiring extension or hybrid domain swing-out. mAb13 and SG/19 bind to the βI domain and βI–hybrid domain interface, respectively, inhibit, and stabilize the closed conformation of the headpiece. The effects of the antibodies on cell adhesion to fibronectin substrates suggest that the extended-open conformation of α5β1 is adhesive and that the extended-closed and bent-closed conformations are nonadhesive. The functional effects and binding sites of antibodies and fibronectin were consistent with their ability in binding to α5β1 on cell surfaces to cross-enhance or inhibit one another by competitive or noncompetitive (allosteric) mechanisms.
National Acad Sciences