Receptor activator of nuclear factor kappa B ligand antagonists inhibit tissue inflammation and bone loss in experimental periodontitis
H Yuan, R Gupte, S Zelkha… - Journal of clinical …, 2011 - Wiley Online Library
H Yuan, R Gupte, S Zelkha, S Amar
Journal of clinical periodontology, 2011•Wiley Online LibraryAim The purpose of this study was to assess the role of anti‐bone resorptive agents and an
anti‐inflammatory compound in murine Porphyromonas gingivalis (P. gingivalis)‐induced
periodontitis. Material and Methods Six randomly assigned groups were administered
vehicle (saline, control)(n= 6), P. gingivalis infection only (untreated)(n= 6), human‐Fc (n=
4), K avain (n= 6), OPG‐Fc (n= 6) and Receptor activator of nuclear factor‐kappa B (RANK)‐
Fc (n= 6) intraperitoneally at day 0, 3 and 7. Animals were euthanized on day 10 and …
anti‐inflammatory compound in murine Porphyromonas gingivalis (P. gingivalis)‐induced
periodontitis. Material and Methods Six randomly assigned groups were administered
vehicle (saline, control)(n= 6), P. gingivalis infection only (untreated)(n= 6), human‐Fc (n=
4), K avain (n= 6), OPG‐Fc (n= 6) and Receptor activator of nuclear factor‐kappa B (RANK)‐
Fc (n= 6) intraperitoneally at day 0, 3 and 7. Animals were euthanized on day 10 and …
Aim
The purpose of this study was to assess the role of anti‐bone resorptive agents and an anti‐inflammatory compound in murine Porphyromonas gingivalis (P. gingivalis)‐induced periodontitis.
Material and Methods
Six randomly assigned groups were administered vehicle (saline, control) (n = 6), P. gingivalis infection only (untreated) (n = 6), human‐Fc (n = 4), Kavain (n = 6), OPG‐Fc (n = 6) and Receptor activator of nuclear factor‐kappa B (RANK)‐Fc (n = 6) intraperitoneally at day 0, 3 and 7. Animals were euthanized on day 10 and subjected to comprehensive histomorphometric analysis. To capture the progress of inflammation, serum samples were collected at days 0, 3, 7 and 10 for levels of pro‐inflammatory cytokines.
Results
Compared with control group, OPG‐Fc, RANK‐Fc and Kavain treatment showed significant bone loss reduction with OPG‐Fc performing better than RANK‐Fc or Kavain. Epithelial down‐growth showed significant reduction in treatment groups with OPG‐Fc performing better than RANK‐Fc or Kavain. Finally, Kavain, OPG‐Fc and RANK‐Fc‐treated mice displayed reduced inflammatory cell counts and cytokine expression particularly at day 7 postinfection.
Conclusions
RANKL antagonists and Kavain effectively reduced alveolar bone loss in P. gingivalis‐induced periodontitis in our mice model. Compared with RANK‐Fc, Kavain‐treated animals showed milder improvement of bone and connective tissue inflammation. Therapeutic implications in the prevention of periodontal bone loss are discussed.
Wiley Online Library