Species-specific binding of sperm proteins to the extracellular matrix (zona pellucida) of the egg.
DM Hardy, DL Garbers - Journal of Biological Chemistry, 1994 - ASBMB
The zona pellucida is an extracellular matrix surrounding the mammalian egg where
species-specific gamete recognition and signaling occur. Pig zona pellucida were isolated
in large amounts and used as an affinity matrix for detergent-solubilized, biotinylated
membrane proteins of pig spermatozoa. On non-reducing SDS-polyacrylamide gel
electrophoresis, specifically bound sperm proteins migrated with M (r) 170,000, 150,000,
130,000, 56,000, and 50,000 (p50). Disulfide bond reduction separated each of the M (r) …
species-specific gamete recognition and signaling occur. Pig zona pellucida were isolated
in large amounts and used as an affinity matrix for detergent-solubilized, biotinylated
membrane proteins of pig spermatozoa. On non-reducing SDS-polyacrylamide gel
electrophoresis, specifically bound sperm proteins migrated with M (r) 170,000, 150,000,
130,000, 56,000, and 50,000 (p50). Disulfide bond reduction separated each of the M (r) …