LRH-1 and PTF1-L coregulate an exocrine pancreas-specific transcriptional network for digestive function

SR Holmstrom, T Deering, GH Swift… - Genes & …, 2011 - genesdev.cshlp.org
SR Holmstrom, T Deering, GH Swift, FJ Poelwijk, DJ Mangelsdorf, SA Kliewer
Genes & development, 2011genesdev.cshlp.org
We have determined the cistrome and transcriptome for the nuclear receptor liver receptor
homolog-1 (LRH-1) in exocrine pancreas. Chromatin immunoprecipitation (ChIP)-seq and
RNA-seq analyses reveal that LRH-1 directly induces expression of genes encoding
digestive enzymes and secretory and mitochondrial proteins. LRH-1 cooperates with the
pancreas transcription factor 1-L complex (PTF1-L) in regulating exocrine pancreas-specific
gene expression. Elimination of LRH-1 in adult mice reduced the concentration of several …
We have determined the cistrome and transcriptome for the nuclear receptor liver receptor homolog-1 (LRH-1) in exocrine pancreas. Chromatin immunoprecipitation (ChIP)-seq and RNA-seq analyses reveal that LRH-1 directly induces expression of genes encoding digestive enzymes and secretory and mitochondrial proteins. LRH-1 cooperates with the pancreas transcription factor 1-L complex (PTF1-L) in regulating exocrine pancreas-specific gene expression. Elimination of LRH-1 in adult mice reduced the concentration of several lipases and proteases in pancreatic fluid and impaired pancreatic fluid secretion in response to cholecystokinin. Thus, LRH-1 is a key regulator of the exocrine pancreas-specific transcriptional network required for the production and secretion of pancreatic fluid.
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