[HTML][HTML] Defining the topology of integrin α5β1-fibronectin interactions using inhibitory anti-α5 and anti-β1 monoclonal antibodies: Evidence that the synergy sequence …
The high affinity interaction of integrin α5β1 with the central cell binding domain (CCBD) of
fibronectin requires both the Arg-Gly-Asp (RGD) sequence (in the 10th type III repeat) and a
second site (in the adjacent 9th type III repeat) which synergizes with RGD. We have
attempted to map the fibronectin binding interface on α5β1 using monoclonal antibodies
(mAbs) that inhibit ligand recognition. The binding of two anti-α5 mAbs (P1D6 and JBS5) to
α5β1 was strongly inhibited by a tryptic CCBD fragment of fibronectin (containing both …
fibronectin requires both the Arg-Gly-Asp (RGD) sequence (in the 10th type III repeat) and a
second site (in the adjacent 9th type III repeat) which synergizes with RGD. We have
attempted to map the fibronectin binding interface on α5β1 using monoclonal antibodies
(mAbs) that inhibit ligand recognition. The binding of two anti-α5 mAbs (P1D6 and JBS5) to
α5β1 was strongly inhibited by a tryptic CCBD fragment of fibronectin (containing both …