[HTML][HTML] Identification of Clinically Significant Tumor Antigens by Selecting Phage Antibody Library on Tumor Cells in Situ Using Laser Capture Microdissection* S
W Ruan, A Sassoon, F An, JP Simko, B Liu - Molecular & Cellular …, 2006 - ASBMB
Much work has been done to develop tumor-targeting antibodies by selecting a phage
antibody library on cancer cell lines. However, when tumor cells are removed from their
natural environment, they may undergo genetic and epigenetic changes yielding different
surface antigens than those seen in actual cases of cancer. We developed a strategy that
allows selection of phage antibodies against tumor cells in situ on both fresh frozen and
paraffin-embedded tissues using laser capture microdissection. By restricting antibody …
antibody library on cancer cell lines. However, when tumor cells are removed from their
natural environment, they may undergo genetic and epigenetic changes yielding different
surface antigens than those seen in actual cases of cancer. We developed a strategy that
allows selection of phage antibodies against tumor cells in situ on both fresh frozen and
paraffin-embedded tissues using laser capture microdissection. By restricting antibody …