[HTML][HTML] Plasma creatinine determination in mice and rats: an enzymatic method compares favorably with a high-performance liquid chromatography assay

A Keppler, N Gretz, R Schmidt, HM Kloetzer… - Kidney international, 2007 - Elsevier
A Keppler, N Gretz, R Schmidt, HM Kloetzer, HJ Gröne, B Lelongt, M Meyer, M Sadick, J Pill
Kidney international, 2007Elsevier
The use of the colorimetric Jaffé method for the measurement of creatinine in mouse and rat
plasma has been criticized as prior studies have shown a dramatic overestimation. We
compared a colorimetric picric acid, an enzymatic, and a high-performance liquid
chromatography (HPLC) method to assess their appropriateness for routine measurements
of creatinine in plasma of healthy and diseased mice (n= 61) and rats (n= 56). For the
colorimetric Jaffé method a pronounced overestimation is confirmed. Additionally the …
The use of the colorimetric Jaffé method for the measurement of creatinine in mouse and rat plasma has been criticized as prior studies have shown a dramatic overestimation. We compared a colorimetric picric acid, an enzymatic, and a high-performance liquid chromatography (HPLC) method to assess their appropriateness for routine measurements of creatinine in plasma of healthy and diseased mice (n=61) and rats (n=56). For the colorimetric Jaffé method a pronounced overestimation is confirmed. Additionally the method showed interference with hemoglobin already in a very low, non-visible concentration range in rat plasma. The enzymatic measurement demonstrated a hemoglobin interference in mice, only when hemolysis was visible. The comparison between HPLC and the enzymatic measurement gave a good agreement between both methods in both species. Therefore the enzymatic method fulfills the requirements for a routine screening test for plasma creatinine in healthy as well as diseased mice and rats over a broad concentration range.
Elsevier