Heart dissection in larval, juvenile and adult zebrafish, Danio rerio

C Singleman, NG Holtzman - JoVE (Journal of Visualized Experiments), 2011 - jove.com
Zebrafish have become a beneficial and practical model organism for the study of embryonic
heart development (see recent reviews1-6), however, work examining post-embryonic
through adult cardiac development has been limited7-10. Examining the changing
morphology of the maturing and aging heart are restricted by the lack of techniques
available for staging and isolating juvenile and adult hearts. In order to analyze heart
development over the fish's lifespan, we dissect zebrafish hearts at numerous stages and …