High-resolution three-dimensional imaging of large specimens with light sheet–based microscopy
PJ Verveer, J Swoger, F Pampaloni, K Greger… - Nature …, 2007 - nature.com
Nature methods, 2007•nature.com
We report that single (or selective) plane illumination microscopy (SPIM), combined with a
new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding
that of confocal fluorescence microscopy in large samples. We demonstrate this by imaging
large living multicellular specimens obtained in a three-dimensional cell culture. The ability
to rapidly image large samples at high resolution with minimal photodamage provides new
opportunities especially for the study of subcellular processes in large living specimens.
new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding
that of confocal fluorescence microscopy in large samples. We demonstrate this by imaging
large living multicellular specimens obtained in a three-dimensional cell culture. The ability
to rapidly image large samples at high resolution with minimal photodamage provides new
opportunities especially for the study of subcellular processes in large living specimens.
Abstract
We report that single (or selective) plane illumination microscopy (SPIM), combined with a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal fluorescence microscopy in large samples. We demonstrate this by imaging large living multicellular specimens obtained in a three-dimensional cell culture. The ability to rapidly image large samples at high resolution with minimal photodamage provides new opportunities especially for the study of subcellular processes in large living specimens.
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