Selective transfer of exosomes from oligodendrocytes to microglia by macropinocytosis

D Fitzner, M Schnaars, D Van Rossum… - Journal of cell …, 2011 - journals.biologists.com
D Fitzner, M Schnaars, D Van Rossum, G Krishnamoorthy, P Dibaj, M Bakhti, T Regen
Journal of cell science, 2011journals.biologists.com
The transfer of antigens from oligodendrocytes to immune cells has been implicated in the
pathogenesis of autoimmune diseases. Here, we show that oligodendrocytes secrete small
membrane vesicles called exosomes, which are specifically and efficiently taken up by
microglia both in vitro and in vivo. Internalisation of exosomes occurs by a macropinocytotic
mechanism without inducing a concomitant inflammatory response. After stimulation of
microglia with interferon-γ, we observe an upregulation of MHC class II in a subpopulation of …
The transfer of antigens from oligodendrocytes to immune cells has been implicated in the pathogenesis of autoimmune diseases. Here, we show that oligodendrocytes secrete small membrane vesicles called exosomes, which are specifically and efficiently taken up by microglia both in vitro and in vivo. Internalisation of exosomes occurs by a macropinocytotic mechanism without inducing a concomitant inflammatory response. After stimulation of microglia with interferon-γ, we observe an upregulation of MHC class II in a subpopulation of microglia. However, exosomes are preferentially internalised in microglia that do not seem to have antigen-presenting capacity. We propose that the constitutive macropinocytotic clearance of exosomes by a subset of microglia represents an important mechanism through which microglia participate in the degradation of oligodendroglial membrane in an immunologically ‘silent’ manner. By designating the capacity for macropinocytosis and antigen presentation to distinct cells, degradation and immune function might be assigned to different subtypes of microglia.
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