[HTML][HTML] Understanding splicing regulation through RNA splicing maps

JT Witten, J Ule - Trends in Genetics, 2011 - cell.com
Trends in Genetics, 2011cell.com
Alternative splicing is a highly regulated process that greatly increases the proteome
diversity and plays an important role in cellular differentiation and disease. Interactions
between RNA-binding proteins (RBPs) and pre-mRNA are the principle regulator of splicing
decisions. Findings from recent genome-wide studies of protein–RNA interactions have
been combined with assays of the global effects of RBPs on splicing to create RNA splicing
maps. These maps integrate information from all pre-mRNAs regulated by single RBPs to …
Alternative splicing is a highly regulated process that greatly increases the proteome diversity and plays an important role in cellular differentiation and disease. Interactions between RNA-binding proteins (RBPs) and pre-mRNA are the principle regulator of splicing decisions. Findings from recent genome-wide studies of protein–RNA interactions have been combined with assays of the global effects of RBPs on splicing to create RNA splicing maps. These maps integrate information from all pre-mRNAs regulated by single RBPs to identify the global positioning principles guiding splicing regulation. Recent studies using this approach have identified a set of positional principles that are shared between diverse RBPs. Here, we discuss how insights from RNA splicing maps of different RBPs inform the mechanistic models of splicing regulation.
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