[HTML][HTML] DNA substrate length and surrounding sequence affect the activation-induced deaminase activity at cytidine
K Yu, FT Huang, MR Lieber - Journal of Biological Chemistry, 2004 - ASBMB
Activation-induced deaminase (AID) is required for both immunoglobulin class switch
recombination and somatic hypermutation. AID is known to deaminate cytidines in single-
stranded DNA, but the relationship of this step to the class switch or somatic hypermutation
processes is not entirely clear. We have studied the activity of a recombinant form of the
mouse AID protein that was purified from a baculovirus expression system. We find that the
length of the single-stranded DNA target is critical to the action of AID at the Cs positioned …
recombination and somatic hypermutation. AID is known to deaminate cytidines in single-
stranded DNA, but the relationship of this step to the class switch or somatic hypermutation
processes is not entirely clear. We have studied the activity of a recombinant form of the
mouse AID protein that was purified from a baculovirus expression system. We find that the
length of the single-stranded DNA target is critical to the action of AID at the Cs positioned …